刘敏

生物工程专业：硕士生导师

生物学专业：硕士生导师

生物与医药专业（生物工程领域）：硕士生导师

刘敏，工学博士，副教授，硕士生导师。2013年6月毕业于浙江大学，获得生物化工博士学位。2014年5月进入awc777万象城娱乐官网工作至今。以第一或通讯作者在ACS synthetic biology、Chemical Communications、Journal of Agricultural and Food Chemistry、Microbial Cell Factories、Journal of Biotechnology等本领域主流学术期刊发表SCI论文多篇。作为项目负责人主持国家自然科学基金、上海市自然科学基金、中国博士后基金面上项目、中央高校基本科研业务项目等多项国家及省部级研究项目。

研究方向：

利用合成生物学原理和技术方法，研究和开发微生物细胞工厂，涉及品种包括萜类化合物、氨基酸及其衍生物、吲哚类生物碱等。

教育/工作经历

2008/9-2013/6    浙江大学化工系            生物化工博士 

2004/9-2008/6    中国矿业大学生物工程系    生物工程学士

2021/7-至今         awc777万象城娱乐官网  副教授

2016/10-2021/6   awc777万象城娱乐官网   讲师

2014/5-2016/9    awc777万象城娱乐官网   博士后 

主持或参加的科研项目或基金：

1.  上海市自然科学基金面上项目（21ZR1417200），2021-2023，主持

2．上海市自然科学基金探索项目（19ZR1473000），2019-2022，主持

3．国家自然科学基金青年项目（31500043），2016-2018，主持

4．中国博士后科学基金面上项目（2014M561427），2014-2016，主持

5．生物反应器工程国家重点实验室开放课题，  2018-2020，主持

6．中央高校基本科研业务费（222201414019），2014-2016，主持

7．企业委托项目，2019-2020，主持

8．企业委托项目，2016-2018，主持

9. 国家自然科学基金面上项目（21776075），2018-2021，参与

10. 国家自然科学基金青年项目（31500066），2016-2018，参与

教学：

本科生专业必修课《生物化学》

本科生公选课《食品安全与饮食健康》

研究生专业核心课《分子生物学进展》（全英文）

研究生专业选修课《人工智能与生物智造》

代表性论文（*通讯作者）：

1. Min Liu, Yang-Chen Lin, Jiao-Jiao Guo, Meng-Meng Du, Xinyi Tao, Bei Gao, Ming Zhao, Yushu Ma, Feng-Qing Wang, Dong-Zhi Wei, High-level production of sesquiterpene patchoulol in Saccharomyces cerevisiae, ACS SYNTH. BIOL., 10 (1), 158-172, 2021.

2. Min Liu, Jiali Lou, Jiali Gu, Xiao-Mei Lyu, Feng-Qing Wang, Dong-Zhi Wei, Increasing L-homoserine production in Escherichia coli by engineering the central metabolic pathways. J. Biotechnol.,2020, 1-7.

3. Ke Liu, Yang Gao, Zhen-Hai Li, Min Liu *, Feng-Qing Wang, Dong-Zhi Wei, CRISPR-Cas12a assisted precise genome editing of Mycolicibacterium neoaurum. New Biotechnol. 2022, 66: 61–69.

4. Bin Ma#, Min Liu#（共一）, Zhen-Hai Li, Xinyi Tao,Dong-Zhi Wei, Feng-Qing Wang. Significantly Enhanced Production of Patchoulol in Metabolically Engineered Saccharomyces cerevisiae. J. Agric. Food Chem., 2019, 67(31), 8590-8598.

5. Min Liu, Liang-Bin Xiong, Xin-Yi Tao, Feng-Qing Wang, Dong-Zhi Wei.Metabolic Adaptation of Mycobacteria in the Catabolism of Sterols for Producing Important Steroid Intermediates. J. Agric. Food Chem., 2018, 66; 12141-12150.

6. Min Liu, Liang-Bin Xiong, Xin-Yi Tao, Qing-Hai Liu, Feng-Qing Wang, Dong-Zhi Wei. Integrated transcriptome and proteome studies reveal the underlying mechanisms for sterol catabolism and steroid production in Mycobacterium neoaurumbum.J. Agric. Food Chem., 2018, 66; 9147-9157.

7. Min Liu, Zhan-Tao Zhu, Xin-Yi Tao, Feng-Qing Wang, Dong-Zhi Wei. RNA-Seq analysis uncovers non-coding small RNA system of Mycobacterium neoaurum in the metabolism of sterols to accumulate steroid intermediates. Microb. Cell Fact.2016, 15:64.

8. Zhen-Hai Li, Hao Meng, Bin Ma, Xinyi Tao, Min Liu*, Feng-Qing Wang, Dong-Zhi Wei. Immediate, multiplexed and sequential genome engineering facilitated by CRISPR/Cas9 in Saccharomyces cerevisiae. J IND MICROBIOL BIOT, 2019, 47, DOI: 10.1007/s10295-019-02251-w

9. Jiali Lou, Min Liu*, Jiali Gu, Qinghai Liu, Li Zhao, Yushu Ma, Dong-Zhi Wei, Metagenomic sequencing reveals microbial gene catalogue of phosphinothricin-utilized soils in South China. Gene, 2019, 711:143942.

10. Xin-Bin Liu#, Min Liu#（共一）, Xin-Yi Tao, Zhong-Xi Zhang, Feng-Qing Wang, Dong-Zhi Wei. Metabolic engineering of Pichia pastoris for the production of dammarenediol-II. J. Biotechnol. 2015, 216: 47-55.

11. Min Liu, Zhan-Tao Zhu, Xin-Yi Tao, Feng-Qing Wang, Dong-Zhi Wei. Single nucleotide polymorphism analysis for the production of valuable steroid intermediates in Mycobacterium neoaurum. Biotechnol. Lett. 2016, 38:1881-1892.

12. Min Liu, Jiali Gu, Wenping Xie, Hongwei Yu. Directed co-evolution of an endoglucanase and a beta-glucosidase in Escherichia coli by a new high throughput screening method. Chem. Commun. 2013, 49: 7219-7221.

13. Min Liu, Lidan Ye, Hongwei Yu. Directed Coevolution of Two Cellulosic Enzymes in Escherichia coli Based on Their Synergistic Reactions. Protein Engineering, 2017, 255-267, DOI: 10.1007/978-1-4939-7366-8_15

14. Min Liu, Hongwei Yu. Cocktail production of an endo-β-xylanase and a β-glucosidase from Trichoderma reesei QM 9414 in Escherichia coli. Biochem. Eng. J. 2012, 68: 1-6.

15. Min Liu, Hongwei Yu. Co-production of a whole cellulase system in Escherichia coli. Biochem. Eng. J. 2012, 69: 204-210. 

16. Min Liu, Wenping Xie, Haoming Xu, Jiali Gu, Xiaomei Lv, Hongwei Yu, Lidan Ye. Directed evolution of an exoglucanase facilitated by a co-expressed β-glucosidase and construction of a whole engineered cellulase system in Escherichia coli.Biotechnol. Lett.2014, 36: 1801-1807.

17. Jiali Gu, Min Liu, Wenping Xie, Fei Guo, Hongwei Yu, Virtual screening of mandelate racemase mutants with enhanced activity based on binding energy in the transition state. Enzyme Microb. Tech. 2014, 55: 121-127.

18. Guo-Song Liu, Tian Li, Wei Zhou, Min Jiang, Xin-Yi Tao, Min Liu, Ming Zhao, Yu-Hong Ren, Bei Gao, Feng-Qing Wang, Dong-Zhi Wei. The yeast peroxisome: A dynamic storage depot and subcellular factory for squalene overproduction. Metabolic Engineering, 2020. DOI: 10.1016/j.ymben.2019.11.001

19. Zhen-Hai Li, Min Liu, Feng-Qing Wang, Dong-Zhi Wei.Cpf1-assisted efficient genomic integration of in vivo assembled DNA parts in Saccharomyces cerevisiae. Biotechnol. Lett.2018, 1-9.

20. Zhen-Hai Li, Min Liu, Xiao-Mei Lyu, Feng-Qing Wang, Dong-Zhi Wei. CRISPR/Cpf1 facilitated large fragment deletion in Saccharomyces cerevisiae. J. Basic Microb., 2018, 1-5.

21. Hao-Hao Liu, Li-Qin Xu, Kang Yao, Liang-Bin Xiong, Xin-Yi Tao, Min Liu, Feng-Qing Wang, Dong-Zhi Wei.Engineered 3-Ketosteroid 9alfa-Hydroxylases in Mycobacterium neoaurum: an Efficient Platform for Production of SteroidDrugs. Appl. Environ. Microb. 2018, 84, e02777-17.

22. Wenping Xie, Min Liu, Xiaomei Lv, Jiali Gu, Wenqiang Lu, Hongwei Yu. Construction of a controllable β-carotene biosynthetic pathway by decentralized assembly method in Saccharomyces cerevisiae. Biotechnol. Bioeng. 2014, 111: 125-133.

23. Xiaomei Lv, Jiali Gu, Fan Wang, Wenping Xie, Min Liu, Lidan Ye, Hongwei Yu, Combinatorial pathway optimization in Escherichia coli by directed co-evolution of rate-limiting enzymes and modular pathway engineering. Biotechnol. Bioeng. 2016, DOI: 10.1002/bit.26034.

发明专利：

1. 刘敏，王风清，魏东芝，林阳琛，一种广藿香醇生产酵母菌株及其构建方法，中国，CN 112175848 A

2. 于洪巍，王丹，王珏，王博，刘敏，磁性共价固定化酶载体的制备方法，中国，ZL201110201473.4。

联系方式：

上海市徐汇区梅陇路130号 

邮编：200237

办公室：实验十四楼鲁华生物技术研究所401室

微信：13585687359

电话：021-64253287

邮箱：lmin@ecust.edu.cn